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重組人骨形態發生蛋白7(rHuBMP-7)
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  • 廠商性質:經銷商
  • 更新時間:2025-05-02
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CYT-333重組人骨形態發生蛋白7(rHuBMP-7) 2μg/10μg/1mg Prospec

 

 

 Background :
 BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

 
Description :
 Recombinant Human BMP-7 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton.
BMP-7 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.

 
Solubility:
 It is recommended to reconstitute the lyophilized rHuBMP-7 in 40mM acetic acid not less than 100µg/ml .

 
Stability:
 
 Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-7 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Applications:
 
1.Molecular standard (Western, ELISA) in studying secreted BMP-7
2.Preparing antibodies for BMP-7 monomer.
3.Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-7 .

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm
2. Analysis by RP-HPLC, using a standard solution of BMP-7 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


 佰易聚生物商城提供美國光譜醫學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!
 

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