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DSP （Lomant's Reagent）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

DSP （Lomant's Reagent）現(xiàn)貨供應(yīng)

DSP （Lomant's Reagent）品牌介紹

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DSP （Lomant's Reagent）僅用于科研

Thermo Scientific Pierce DSP （Lomant's Reagent） is an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm.

DSP contains an amine-reactive N-hydroxysuccinimide （NHS） ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS, the non-cleavable analog of DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

DSP Highlights

• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
• Cleavable crosslinker allows separation of crosslinked products
• DSP crosslinker is membrane permeable, so intracellular crosslinking can be done

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