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EZ-Link （tm） Biotin-LC-Hydrazide介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

EZ-Link （tm） Biotin-LC-Hydrazide現(xiàn)貨供應

EZ-Link （tm） Biotin-LC-Hydrazide品牌介紹

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EZ-Link （tm） Biotin-LC-Hydrazide僅用于科研

Thermo Scientific EZ-Link Hydrazide-Biotin is the shortest and simplest hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

Thermo Scientific EZ-Link Hydrazide-LC-Biotin is a mid-length, simple, hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

These biotin hydrazide reagents enable simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.

Highlights:

• Label glycoproteins with biotin to facilitate immobilization, purification or detection
• Attaches a biotin label to antibodies through carbohydrate modifications in the Fc region
• Biotin hydrazides react with oxidized carbohydrates （aldehydes） at pH 4 to 6 to attach biotin through stable hydrazone bonds
• Biotin hydrazides can be used to specifically label cell surface glycoproteins